【摘 要】
：
Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has become a global health threat since December 2019,and there is still no highly effective drug to control the pandemic.To facil-itate drug target identification for drug development,studies on
【机 构】
：
Shanghai Center for Systems Biomedicine,Key Laboratory of Systems Biomedicine(Ministry of Education)
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Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has become a global health threat since December 2019,and there is still no highly effective drug to control the pandemic.To facil-itate drug target identification for drug development,studies on molecular mechanisms,such as SARS-CoV-2 protein interactions,are urgently needed.In this study,we focused on Nsp2,a non-structural protein with largely unknown function and mechanism.The interactome of Nsp2 was revealed through the combination of affinity purification mass spectrometry(AP-MS)and sta-ble isotope labeling by amino acids in cell culture(SILAC),and 84 proteins of high-confidence were identified.Gene ontology analysis demonstrated that Nsp2-interacting proteins are involved in several biological processes such as endosome transport and translation.Network analysis generated two clusters,including ribosome assembly and vesicular transport.Bio-layer interfer-ometry(BLI)assay confirmed the bindings between Nsp2-and 4-interacting proteins,i.e.STAU2(Staufen2),HNRNPLL,ATP6V1B2,and RAP1GDS1(SmgGDS),which were randomly selected from the list of 84 proteins.Our findings provide insights into the Nsp2-host interplay and indicate that Nsp2 may play important roles in SARS-CoV-2 infection and serve as a potential drug target for anti-SARS-CoV-2 drug development.
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