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目的 构建甲型流感病毒HA受体结合特异性检测方法 ,了解当前各亚型流行株受体结合特性.方法以辣根过氧化物酶标记去唾液酸胎球蛋白(Fet-HRP),分别用α-2,3-(N)-唾液酸转移酶和α-2,6-(N)-唾液酸转移酶处理,合成3-Fet-HRP、6-Fet-HRP特异性受体拟合蛋白.分别包被怀槐凝集素(MAA)、接骨木凝集素(SNA)和各亚型流感病毒行ELISA试验进行受体特异性鉴定.结果 该检测方法成功建立,各亚型与3-Fet-HRP、6-Fet-HRP均有一定结合.H1N1、H3N2亚型结合6-Fet-HRP的能力高于3-Fet-HRP,禽流感H5N6亚型偏向于与α-2,3受体结合,H7N9亚型对2种蛋白结合能力无明显差异.结论 该方法对了解当前流感流行株生物学特性和未来新型毒株的传播方向和风险提供科学预测.“,”Objective To develop a method for detection of the receptor-binding property of influenza A virus HA and the receptor of the current pandemic influenza viruses. Methods The asialofetuin was labeled with HRP andα-2,3-(N)andα-2,6-(N)sialytransferases were used to process the labeled asiolofetuin into two special receptor analogs. ELISA was used to test the receptor-binding property of coated MAA, SNA and influenza viruses of four subtypes with the receptor analogs. Results This detection method was established successfully. Viruses of all subtypes could somewhat bind with both 3-Fet-HRP and 6-Fet-HRP. H1N1 and H3N2 recognized 6-Fet-HRP in preference to 3-Fet-HRP, H5N6 preferred to bind withα-2, 3 receptor, and H7N9 bound with the both receptors with no significant difference. Conclusion This method could detect the biological property of pandemic influenza virus, and provide scientific prediction of the transmission direction and risk of new virus in the future.