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细胞滋养层细胞和肿瘤细胞的侵入行为具有惊人的相似性，其中基质金属蛋白酶（ＭＭＰｓ）是滋养层细胞和肿瘤细胞侵入的非常重要的介导因素．近来，ＭＭＰｓ家族的一个新成员── ＭＭＰ－２８被克隆并确定下来，采用反转录－聚合酶链式反应（ＲＴ－ＰＣＲ）、酶谱分析和Ｎｏｒｔｈｅｒｎ ｂｌｏｔ等方法，检测了妊娠早期细胞滋养层细胞和绒毛膜癌细胞系ＪＥＧ－３细胞中ＭＭＰ－２８的基因表达和蛋白分泌情况．ＲＴ－ＰＣＲ显示，细胞滋养层细胞和ＪＥＧ－３细胞均有ＭＭＰ－２８ ｍＲＮＡ的表达；Ｎｏｒｔｈｅｒｎ ｂｌｏｔ研究显示，ＪＥＧ－３细胞中ＭＭＰ－２８ ｍＲＮＡ的表达强于细胞滋养层细胞中ＭＭＰ－２８ ｍＲＮＡ的表达，具有时间依赖关系；酶谱分析显示，ＪＥＧ－３细胞中ＭＭＰ－２８蛋白分泌活性高于细胞滋养层细胞中ＭＭＰ－２８蛋白分泌活性，呈时间依赖关系，这与ＭＭＰ－２８的基因表达结果相一致．进一步证明了ＭＭＰ－２８可能在正常妊娠和肿瘤发展等一些与组织重建有关的过程中发挥一定的作用．
There are striking resemblances between the invasive behavior of trophoblast cells and tumor cells, of which matrix metalloproteinases (MMPs) are very important mediators of trophoblast and tumor cell invasion. Recently, a new member of the MMPs family, MMP-28, was cloned and identified. RT-PCR, enzyme-linked immunosorbent assay and Northern blot were used to detect cytotoxity during early gestation Gene expression and protein secretion of MMP-28 in layer cells and choriocarcinoma cell line JEG-3. The expression of MMP-28 mRNA in both cytotrophoblasts and JEG-3 cells was detected by RT-PCR. The results of Northern blot showed that the expression of MMP-28 mRNA in JEG-3 cells was stronger than that in the cytotrophoblast The results of zymogram showed that the secretion activity of MMP-28 in JEG-3 cells was higher than the secretion of MMP-28 in cytotrophoblasts in a time-dependent manner, which was correlated with the expression of MMP-28 Expression consistent. Further evidence that MMP-28 may play a role in some processes related to tissue remodeling, such as normal pregnancy and tumor development.