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实验选用170～210g的雄性Sprague-Dawley大鼠,侧脑室注入秋水仙素80～120μg,48小时后经左心室灌流固定,恒冷箱连续切片,厚5μm,每两个相邻的切片相对应,融表在两组载片上,分别用促肾上腺皮质激素释放因子抗血清(Anti-CRF)和神经降压素抗血清(Anti-NT)孵育,ABC或PAP免疫组织化学反应后,DAB呈色,检查、比较配对切片上免疫反应阳性细胞的位置和形态。结果,在相邻切片上看到卵圆核内有数量不等的位置相同、形态相似的免疫反应阳性细胞,即CRF-NT双标记或两种神经肽共存神经元。本组实验首次发现卵圆核内有CRF-NT共存神经元。
Experimental male Sprague-Dawley rats (170-210g) were injected into the lateral ventricle. Colchicine was injected into the lateral ventricle 80 ~ 120μg. After 48 hours, they were fixed by left ventricular perfusion. The continuous cold box was sliced continuously and the thickness was 5μm. Each two adjacent slices corresponded , Fused on two sets of slides and incubated with Anti-CRF and Anti-NT respectively. After ABC or PAP immunohistochemical reaction, DAB color , Check and compare the location and morphology of immunoreactive cells on the matched sections. As a result, a number of immunoreactive positive cells in the oval nucleus in the same number in the same position with similar shapes were observed on the adjacent sections, that is, CRF-NT double-labeled neurons or two neuropeptide co-existing neurons. This experiment for the first time found in the oval nucleus CRF-NT co-existing neurons.