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目的观察脂多糖(LPS)对成纤维细胞基质金属蛋白酶(MMPs)及其抑制物金属蛋白酶组织抑制因子(TIMPs)表达的变化,探讨伤口感染时LPS对伤口愈合的可能影响。方法将体外培养的人成纤维细胞加入不同浓度的LPS(10、100、1000μg/ml)后,用荧光标记的明胶酶谱法和反向酶谱法测定培养液中MMPs及TIMPs表达的变化,并用电泳分析软件对黑色条带(MMPs)或亮色条带(TIMPs)的面积及密度进行半定量分析。结果LPS可以上调成纤维细胞的MMP-2、TIMP-1和TIMP-2的表达,且TIMPs增加的程度大于MMPs。10、100、1000μg/ml的LPS使MMP-2分别增加为基值的1.3、1.7、1.6倍,TIMP-1分别为3、4.5、3.5倍,TIMP-2分别为3、6、5.6倍。结论LPS在伤口愈合过程中成纤维细胞表达MMPs和TIMPs的环节上,对伤口愈合没有明显的不利影响。
Objective To investigate the changes of matrix metalloproteinases (MMPs) and inhibitor of metalloproteinase (TIMPs) in fibroblasts induced by lipopolysaccharide (LPS), and to explore the possible effect of LPS on wound healing during wound infection. Methods Human fibroblasts cultured in vitro were cultured in different concentrations of LPS (10,100,1000μg / ml), and the changes of MMPs and TIMPs expression in culture medium were determined by fluorescence-labeled gelatin zymography and reverse-phase zymography. The area and density of black bands (MMPs) or bright bands (TIMPs) were semi-quantitatively analyzed by electrophoresis software. Results LPS up-regulated the expression of MMP-2, TIMP-1 and TIMP-2 in fibroblasts, and TIMPs increased more than MMPs. LPS at 10, 100, and 1000μg / ml increased MMP-2 to 1.3, 1.7 and 1.6 times respectively, TIMP-1 and TIMP-1 were 3, Conclusions LPS can express MMPs and TIMPs in fibroblasts during wound healing without significant adverse effects on wound healing.