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目的:研究小檗碱对去卵巢大鼠骨量丢失的作用。方法:以去卵巢诱导大鼠骨质疏松症,3月龄雌性SD大鼠随机分为假手术组、去卵巢组(空白组)、阳性对照组(尼尔雌醇组)及低(10 mg·kg-1)、中(30 mg·kg-1)、高(90 mg·kg-1)剂量小檗碱组,每组10只。12周后,取胫骨近端行不脱钙制片,采用骨组织形态计量学方法测定胫骨中段皮质骨动、静态学参数。双能X射线骨密度仪测量股骨骨密度。结果:与空白组比较,小檗碱能显著增加大鼠胫骨中段松质骨骨小梁面积百分比和骨小梁厚度(P<0.01),减少骨小梁分离度(P<0.05);增加骨形成参数:矿化沉积率(MAR)、骨形成率(BFR/BS、BFR/BV、BFR/TV)(P<0.05或0.01);同时,显著增加去卵巢大鼠股骨骨密度(P<0.05)。对胫骨干骺端松质骨破骨细胞数目及体质量差异无统计学意义。结论:小檗碱对去卵巢大鼠骨量丢失有保护作用,主要是通过促进骨形成、抑制骨吸收、提高骨密度。
Objective: To study the effect of berberine on bone loss in ovariectomized rats. Methods: Osteoporosis was induced in ovariectomized rats. Three-month-old female Sprague-Dawley rats were randomly divided into sham operation group, ovariectomized group (n = 10), positive control group (nilestriol) and low · Kg-1), middle (30 mg · kg-1) and high (90 mg · kg-1) berberine groups. Twelve weeks later, the non-decalcified tablets were taken from the proximal tibia, and the cortical bone dynamic and static parameters were measured by histomorphometry. Dual energy X-ray absorptiometry measures femoral bone mineral density. Results: Compared with the blank group, berberine could significantly increase trabecular bone area and trabecular thickness (P <0.01), decrease trabecular separation (P <0.05), increase bone mass (P <0.05 or 0.01). At the same time, BMD of femur in ovariectomized rats was significantly increased (P <0.05), and the parameters of formation mineralization rate (MAR), bone formation rate (BFR / BS, BFR / BV, BFR / TV) ). The tibia metaphyseal cancellous bone osteoclasts number and body weight difference was not statistically significant. Conclusion: Berberine has a protective effect on bone loss in ovariectomized rats, mainly through promoting bone formation, inhibiting bone resorption and increasing bone density.