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目的通过对SEMA3B基因在原发性肝细胞癌(HCC)组织中表达的检测,探讨该基因与HCC发生机制的关系。方法采用逆转录-聚合酶链反应(RT-PCR)方法检测35例HCC组织及癌旁肝组织SEMA3B mRNA表达水平。结果(1)在所有受检的癌旁肝组织中,SEMA3B mR- NA表达率为85.7%(30/35),明显高于癌组织的表达率20.0%(7/35),差异有统计学意义(P<0.01);(2)合并有肝硬化(20/28)或者乙肝(19/28)的患者,其癌组织中SEMA3B基因的表达缺失率显著高于无肝硬化(8/28)或乙肝阴性(9/28)患者(P<0.05);(3)SEMA3B基因的异常表达情况与年龄、性别的差异无统计学意义(P>0.05),与HCC患者术前肝功能Child-Pugh分级评价和血清AFP的检测值高低无相关(P>0.05);(4)SEMA3B的表达缺失率与TNM分期关系无统计学差异,低分化型癌组织中SEMA3B基因缺失率高于中、高分化型,但差异无统计学意义(P>0.05)。此外,该基因的缺失与肿瘤大小无关(P>0.05)。结论SEMA3B基因在HCC组织中的高频表达缺失说明该基因的失活与HCC的发生密切相关。SEMA3B基因是定位于染色体3p21.3区域的与HCC相关的抑癌基因,并且可能在HCC的发生发展方面起重要作用。
Objective To investigate the expression of SEMA3B gene in primary hepatocellular carcinoma (HCC) and to explore its relationship with the pathogenesis of HCC. Methods The expression of SEMA3B mRNA in 35 HCC tissues and adjacent noncancerous liver tissues was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results (1) The expression of mRNA in SEMA3B was 85.7% (30/35) in all the adjacent non-cancerous liver tissues, which was significantly higher than that in the cancerous tissues (20.0%, 7/35) (P <0.01). (2) In patients with cirrhosis (20/28) or hepatitis B (19/28), the loss of SEMA3B gene expression was significantly higher in patients with cirrhosis (8/28) (P <0.05). (3) The abnormal expression of SEMA3B gene had no significant difference with age and sex (P> 0.05), but was not correlated with Child-Pugh (4) There was no significant difference in the expression of SEMA3B between TNM staging and TNM stage. The loss rate of SEMA3B gene in poorly differentiated carcinoma was higher than that of moderate and high differentiated Type, but the difference was not statistically significant (P> 0.05). In addition, the deletion of this gene was not related to tumor size (P> 0.05). Conclusion The lack of high frequency expression of SEMA3B in HCC suggests that the inactivation of this gene is closely related to the occurrence of HCC. The SEMA3B gene is a HCC-related tumor suppressor gene located in the 3p21.3 region of chromosome, and may play an important role in the development of HCC.