论文部分内容阅读
为了评价大豆抗原蛋白对断奶仔猪有无致敏作用及对肠紧密连接蛋白ZO-1表达的影响,选取70头24日龄断奶仔猪,随机将其分为7组,A组饲喂基础日粮,B组、C组、D组的饲粮中分别按仔猪初始体重添加500、2 000、5 000 mg/kg的β-伴大豆球蛋白,E组、F组、G组的饲粮中按仔猪初始体重分别添加500、2 000、5 000 mg/kg的大豆球蛋白,通过ELISA检测仔猪24及30日龄时血清中IgG及IgE的质量浓度,并于仔猪30日龄时,每组选取5头仔猪放血致死,采集小肠组织,通过免疫组化检测小肠紧密连接蛋白ZO-1的表达情况,并用实时荧光定量PCR测定ZO-1 m RNA的相对表达量。统计学分析结果表明,在仔猪日粮中添加大豆抗原蛋白能刺激断奶仔猪血清IgG和IgE的质量浓度升高(P<0.01),小肠ZO-1蛋白表达和ZO-1 m RNA的相对表达量降低,且大豆抗原蛋白的添加剂量越多,ZO-1表达越低。上述结果表明,β-伴大豆球蛋白和大豆球蛋白能引起仔猪肠道过敏反应,损伤肠道紧密连接;β-伴大豆球蛋白的致敏作用大于大豆球蛋白。
To evaluate the sensitization effect of soybean antigen protein on weaned piglets and the expression of tight junction protein ZO-1, 70 24-day-old weaned piglets were randomly divided into 7 groups. Group A was fed basal diet , Group B, group C and group D were fed with 500, 2, 000 and 5 000 mg / kg of β-conglycinin respectively according to the initial weight of piglet. In group E, group F and group G, The initial weights of piglets were respectively added with 500, 2 000 and 5 000 mg / kg of glycinin. Serum IgG and IgE concentrations of piglets at 24 and 30 days of age were detected by ELISA. At 30 days of age, Five piglets were sacrificed by bloodletting, the small intestine tissues were harvested and the expression of small intestinal tight junction protein ZO-1 was detected by immunohistochemistry. The relative expression of ZO-1 mRNA was detected by real-time fluorescence quantitative PCR. Statistical analysis showed that the addition of soybean antigen protein in piglets could stimulate the increase of serum IgG and IgE concentrations (P <0.01), the expression of ZO-1 protein and the relative expression of ZO-1 mRNA in small intestine Decreased, and the more the additive dose of soybean antigen protein, the lower the expression of ZO-1. The above results show that β-conglycinin and glycinin can cause intestinal anaphylaxis in piglets and impair intestinal tight junctions; β-conglycinin is more sensitized than glycinin.