抗菌肽(Antimicrobial peptides,APMs)是一类对细菌、真菌等微生物及某些昆虫和动植物细胞具有抑制作用的小分子多肽。将碗豆防御素基因Psd1插入甲醇酵母Pichia pastoris的分泌表达质粒pPIC9K中,并将含有目的基因的重组质粒电转化P.pastoris GS115(his4),得到重组酵母GS115/PSD菌株。试验结果表明,重组酵母菌株经扩大培养,诱导表达的菌体被破碎后,酵母悬浮液含量达到5×108细胞/mL时,可以很好地抑制采后柑橘的酸腐病。 Antimicrobial peptides (APMs) are a class of small molecules that inhibit the growth of bacteria, fungi and other microorganisms as well as certain insect, animal and plant cells. The Pdd1 gene of Pichia pastoris was inserted into Pichia pastoris and transformed into P. pastoris GS115 (his4) by electroporation. The recombinant yeast GS115 / PSD strain was obtained. The results showed that after the recombinant yeast strain was expanded and the induced bacterial cells were broken, the acid rot of postharvest citrus could be well inhibited when the yeast suspension content reached 5 × 10 8 cells / mL.