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采用超薄层聚丙烯酰胺凝胶等电聚焦法对血痕Tf亚型进行分型,并调查北京地区223名无血缘关系汉族人群Tf亚型的分布。其基因频率:TfC~10.7354,TfC~20.2377 TfDchi 0.0269。经Hardy-Weinberg吻合度检验,观察值与期望值无显著性差异(ΣX~2=0.9905 df=3 p>0.50)。Tf的个人识别率为0.4020。非父排除率为0.1813。讨论了汉族群体Tf亚型及不同地区,不同人种间Tf分布的情况。到目前为止,室温下保存8个月的血痕仍可进行Tf亚型分型。对实验中质量的控制作了探讨,采用伏时控制电泳条件。
The ultrafine layer polyacrylamide gel isoelectric focusing method was used to classify the Tf subtype of bloodstain and investigate the distribution of Tf subtypes in 223 unrelated Han Chinese population in Beijing. The gene frequency: TfC ~ 10.7354, TfC ~ 20.2377 TfDchi 0.0269. The Hardy-Weinberg test showed that there was no significant difference between the observation and the expected value (ΣX ~ 2 = 0.9905 df = 3 p> 0.50). Tf’s personal identification rate is 0.4020. The non-parent exclusion rate was 0.1813. Tf subfamilies of Han population and distribution of Tf among different ethnic groups and different ethnic groups were discussed. So far, bloodstains preserved for 8 months at room temperature can still be Tf subtype. The control of the quality of the experiment was discussed, the use of volt control electrophoresis conditions.