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继1965年Morel等人用芽分生组织进行组织培养,成功地快速繁殖兰花后,近年来人们对于应用组织培养技术作为园艺植物无性繁殖的一种方法的兴趣日渐增加。好几种植物使用茎尖培养方法进行无性繁殖都已取得了很大进展,这些植物包括石刁柏、菊花、大丁草属、天竺葵属和芋。1976年笔者简略地报道过在试管中少量繁殖菠萝;本文报道用菠萝腋芽和叶作外植体培养时,关于控制形态发生和小植株形成的一些因素的进一步研究。
Following Morel et al.’s organization of tissue culture using shoot meristems in 1965, the interest in applying clonal culture techniques as a means of clonal horticulture has been increasing in recent years following successful propagation of orchids. Much progress has been made in the vegetative propagation of several plants using shoot tip culture, including asparagus, chrysanthemum, gerbera, pelargonium and taro. In 1976, the author briefly reported that pineapples were multiplied in small quantities in test tubes. Further studies on some factors controlling morphogenesis and plantlet formation were reported in this paper when pineapple axillary buds and leaves were used as explants.