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目的:观察大肠癌组织中PTEN缺失和Skp2表达情况,并观察两者与大肠癌临床病理特征和预后的关系,探讨其在大肠癌预后的价值。方法:采用SP方法检测大肠癌组(61例)和对照组(20例)中PTEN和Skp2蛋白的表达,分析其表达与患者临床病理特征和5年生存率的关系,并观察两者之间的相关性。结果:大肠癌组PTEN表达率(72.13%)明显低于对照组(100.00%);大肠癌组Skp2表达率(52.46%)明显高于对照组;PTEN缺失和Skp2的表达均与组织分化程度、淋巴结转移和临床分期呈正相关。PTEN阴性组的5年生存率(31.86%)低于阳性组(83.97%),Skp2阳性组的5年生存率(58.33%)低于阴性组(82.76%)。PTEN和Skp2的表达呈负相关,r=-0.445,P=0.001。结论:PTEN缺失和Skp2表达可能与大肠癌发生、转移和预后有关,联合检测PTEN和Skp2蛋白可作为预测大肠癌发生转移和预后的指标。
OBJECTIVE: To observe the relationship between PTEN deletion and Skp2 expression in colorectal carcinoma and the relationship between them and the clinicopathological features and prognosis of colorectal carcinoma, and to explore its value in the prognosis of colorectal carcinoma. Methods: The expression of PTEN and Skp2 protein in colorectal cancer (61 cases) and control group (20 cases) was detected by SP method. The relationship between the expression of PTEN and Skp2 protein and clinicopathological features and 5-year survival rate was analyzed. Relevance. Results: The expression of PTEN in colorectal cancer group was significantly lower than that in control group (72.13% vs 100.00%). The expression of Skp2 in colorectal cancer group was significantly higher than that in control group (52.46%). PTEN deletion and Skp2 expression were correlated with histological grade, Lymph node metastasis and clinical stage were positively correlated. The 5-year survival rate (31.86%) in PTEN negative group was lower than that in positive group (83.97%). The 5-year survival rate in Skp2 positive group (58.33%) was lower than that in negative group (82.76%). PTEN and Skp2 expression was negatively correlated, r = -0.445, P = 0.001. CONCLUSION: PTEN deletion and Skp2 expression may be related to the occurrence, metastasis and prognosis of colorectal cancer. Combined detection of PTEN and Skp2 protein may be used as an index to predict the metastasis and prognosis of colorectal cancer.