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目的探讨运用多功能超高倍显微分析仪直接湿片观察精子形态的应用价值。方法支原体感染不育症患者156份精液标本,用改良巴氏染色法,1 000倍油镜观察精子形态,同时运用多功能超高倍显微镜直接湿片观察精子形态。结果两种方法对精子形态的评估经统计学分析,正常形态率和各种类型缺陷率差异均无统计学意义(P>0.05)。结论运用多功能超高倍显微分析仪直接观察精子形态是一种简单、快速、有效的方法,值得推广。
Objective To explore the application of multi-function ultra-high magnification microanalysis instrument to directly observe the morphology of sperm. Methods 156 semen specimens of infertile patients were treated with mycoplasma. Sperm morphology was observed with modified Papanicolaou and 1 000 times oil microscope. Morphology of the sperm was observed with a multi-function ultra-high power microscope. Results Evaluation of sperm morphology by two methods There was no significant difference between normal morphology rate and various types of defect rate by statistical analysis (P> 0.05). Conclusions The direct observation of sperm morphology by using multi-functional ultra-high magnification microscopy analyzer is a simple, rapid and effective method that is worth promoting.