论文部分内容阅读
为调查动物源性致病性大肠杆菌氨基糖苷修饰酶耐药基因的携带情况,探讨耐药基因与氨基糖苷类抗生素表型的相关性,对40株源于畜禽的大肠杆菌常用氨基糖苷类抗生素进行药敏试验,用PCR法检测耐药基因aac(3)-Ⅱ、arm A、aac(6’)-Ⅰb。结果表明:40株大肠杆菌在所用氨基糖苷类药物中对卡那霉素的耐药率最高,达57.5%(23/40),其次是链霉素与庆大霉素,耐药率分别为45.0%(18/40)和40.0%(16/40);耐药基因aac(3)-Ⅱ的检出率为17.5%(7/40),其中有6株的耐药表型都为对庆大霉素耐药,arm A的检出率为0,aac(6’)-Ⅰb的检出率为2.5%(1/40)。
In order to investigate the relationship between the resistance genes of aminoglycoside-modifying enzymes of pathogenic Escherichia coli and the phenotypes of aminoglycoside antibiotics, 40 aminoglycosides commonly used in Escherichia coli Antibiotics were tested for susceptibility. The resistance genes aac (3) -Ⅱ, arm A, aac (6 ’) - Ⅰb were detected by PCR. The results showed that 40 strains of Escherichia coli were the most resistant to kanamycin in the aminoglycosides used, accounting for 57.5% (23/40), followed by streptomycin and gentamicin, the rates of resistance were 45.0% (18/40) and 40.0% (16/40), respectively. The resistance rate of aac (3) -Ⅱ was 17.5% (7/40) Gentamicin resistance was detected with a detection rate of arm A of 0 and aac (6 ’) - Ib of 2.5% (1/40).