应用酵母双杂交技术筛选Herp的相互作用蛋白。构建编码Herp的基因HERPUD1真核表达载体HERPUD1plexA,应用MATCHMAKERLexA酵母双杂交系统筛选人胎脑cDNA文库,获得的阳性克隆的插入子为Herp的候选相互作用蛋白质,将Herp与筛选到的相互作用蛋白再一对一回复进行酵母双杂交实验,去除假阳性。对阳性克隆插入子的DNA序列测序,在GenBank中作匹配及生物信息学分析。结果得到其中1个阳性克隆的插入子序列与TEGT基因序列一致,编码蛋白为Baxinhibitor1。得出结论:Herp与Baxinhibitor1相互作用,Baxinhibitor1具有调节凋亡特性,提示Herp可能参与凋亡调节。 Screening Herp Interacting Proteins by Yeast Two - hybrid Technique. To construct the HERPUD1 eukaryotic expression vector HERPUD1plexA, which encodes Herp gene, the human fetal brain cDNA library was screened by MATCHMAKERLexA yeast two-hybrid system. The positive clones were obtained as Herp candidate interacting proteins. Herp and the selected interacting proteins One-on-one response to yeast two-hybrid experiments to remove false positives. The DNA sequence of the positive cloned insert was sequenced and matched in GenBank and bioinformatics analysis. Results The insert of one of the positive clones was consistent with the sequence of TEGT gene. The encoded protein was Baxinhibitor1. Concluded that: Herp and Baxinhibitor1 interaction, Baxinhibitor1 has the characteristics of regulating apoptosis, suggesting that Herp may be involved in apoptosis regulation.