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视网膜新生血管性疾病是一类严重的致盲性疾病,构建与之相关的动物模型是进行该疾病机制研究的重要基础,有利于在临床中更好地预防、诊断和治疗。氧诱导视网膜病变小鼠模型是目前研究视网膜新生血管性疾病最常用的动物模型,此模型构建过程与人类早产儿视网膜新生血管病变的发生、发展极为相似,具有制作方法简单、重复性好等优点。本文重点阐述氧诱导视网膜病变小鼠模型的构建方法及原理,模型相关细胞因子的作用,模型构建的影响因素如动物品种、氧气的百分含量、高氧对母鼠的影响、产后体质量增加量、光照暴露等,并简要介绍模型中常用的量化评估方法,为研究人员准确、有效地构建和评估氧诱导视网膜病变小鼠模型提供帮助。
The retinal neovascular disease is a kind of serious blinding disease. The construction of animal model related with it is an important foundation for the study of the mechanism of the disease, which is beneficial to the prevention, diagnosis and treatment in the clinic. The mouse model of oxygen-induced retinopathy is currently the most commonly used animal model of retinal neovascular disease. The construction of this model is very similar to the occurrence and development of retinal neovascular disease in human premature babies. The method has the advantages of simple production method and good repeatability . This article focuses on the construction of oxygen-induced retinopathy mouse model and principles, model-related cytokines, model building factors such as animal species, the percentage of oxygen, the impact of hyperoxia on female rats, postpartum body mass gain Quantity, exposure to light and so on. The quantitative assessment methods commonly used in the model are briefly introduced to help researchers construct and evaluate the mouse model of oxygen-induced retinopathy accurately and effectively.