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本研究旨在探讨不同白血病细胞系、成人急性髓系白血病Ang-1、Ang-2、Tie-2、Kindlin-2、Kindlin-3的表达情况及其意义。采用RQ-PCR方法检测88例AML患者、9例非肿瘤患者(对照组)以及K562、KG-1a、U937、HL-60、Jurkat细胞株中Ang-1、Ang-2、Tie-2、Kindlin-2、Kindlin-3表达水平,分析阳性率及表达水平在AML患者与对照组间的差异,探讨上述5种基因之间及其与AML分型、预后的关系。结果表明,Ang-1,Ang-2,Kindlin-3在K562、KG-1a、HL-60、U937、Jurkat细胞系中均有表达,Tie-2仅表达于KG-1a、HL-60细胞系中,Kindlin-2表达于K562、KG-1a、HL-60中。5种基因均表达于AML患者及对照组中。Ang-1、Ang-2在起病时白细胞计数较高组的表达水平较高(P<0.001,P=0.001),在伴有t(8;21)和t(15;17)的AML患者中表达均较低(P<0.001,P=0.005)。Ang-1在NCCN预后良好组表达较低(P=0.020);Ang-1低表达组完全缓解率(CR)较高(P=0.027)。Kindlin-2在AML患者中表达水平较低(P=0.010),起病时白细胞计数较高组的表达水平较低(P=0.020),伴有t(8;21)和t(15;17)AML的表达均较高(P=0.016),治疗缓解后Kindlin-2及Kindlin-3表达明显升高(P<0.001,P=0.004)。结论:Ang-1与AML不良预后因素相关;Kindlin-2在AML中低表达,与AML良好预后因素相关,可能是一个预后较好的标志。
This study aimed to investigate the expression of Ang-1, Ang-2, Tie-2, Kindlin-2 and Kindlin-3 in different leukemia cell lines and adult acute myeloid leukemia and its significance. The expression of Ang-1, Ang-2, Tie-2, Kindlin in 88 AML patients, 9 non-neoplastic patients (control group) and K562, KG-1a, U937, HL-60 and Jurkat cell lines were detected by RQ- -2, Kindlin-3 expression levels, the positive rate and expression levels in AML patients and control group differences between the above five genes and their relationship with the classification of AML, prognosis. The results showed that Ang-1, Ang-2 and Kindlin-3 were expressed in K562, KG-1a, HL-60, U937 and Jurkat cell lines respectively. Tie-2 was only expressed in KG- , Kindlin-2 is expressed in K562, KG-1a, HL-60. All five genes were expressed in AML patients and controls. The expression of Ang-1 and Ang-2 was higher in patients with higher leukocyte count at onset (P <0.001, P = 0.001). In AML patients with t (8; 21) and t The expression was lower (P <0.001, P = 0.005). The expression of Ang-1 was lower in patients with good prognosis of NCCN (P = 0.020) and higher in patients with low expression of Ang-1 (P = 0.027). Kindlin-2 had a lower expression level in AML patients (P = 0.010), a lower leukocyte count at onset (P = 0.020), t (8; 21) and t ) AML (P = 0.016). After treatment, the expressions of Kindlin-2 and Kindlin-3 were significantly increased (P <0.001, P = 0.004). Conclusions: Ang-1 is associated with adverse prognostic factors of AML. Kindlin-2 is low in AML, which may be a good marker of prognosis.