目的　建立检测肾综合征出血热 (HFRS)患者血清标本中HV基因的PCR ELISA方法。方法　设计并合成互补于HVS基因片段的标记引物 ,对 10 8例HFRS患者血清进行巢式RT PCR扩增 ,并用酶免方法分析扩增产物。结果　 10 8例患者不同病日采集的血清标本的巢式RT PCR平均检出率仅为 6 2 0 % ,而PCR ELISA平均检出率为 81 5 %。结论　PCR ELISA用于早期HFRS患者的HV基因检测是一种理想的检测方法 Objective To establish a PCR ELISA method for detecting HV gene in serum samples from patients with hemorrhagic fever with renal syndrome (HFRS). Methods Primers complementary to HVS gene fragments were designed and synthesized. The serum of 108 patients with HFRS was amplified by nested RT-PCR, and the amplification products were analyzed by enzyme-free method. Results The average positive detection rate of nested RT PCR for serum samples collected on different days was 108%, while the average detection rate of PCR ELISA was 81.5%. Conclusions PCR ELISA is an ideal method for the detection of HV gene in patients with early HFRS