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显微镜下对抗酸菌的直接计数,是麻风实验研究中的一种基本技术。目前应用的方法大致分为二类:1、用微量吸管或标定载液量的接种环将菌液涂佈于标有直径为0.8～1.13厘米圆环的玻片上。 2、用标定载液量的大头针针头,蘸菌液在玻片上作点状涂佈,即“针头法”。这2种方法虽各有优点,但都不能解决液滴在自然干燥过程中向涂点的中央和边缘浓集的问题,以致细菌的分佈很不均匀。同一涂点不同区域的菌量差异可达10倍以上,如果悬液内组织含量低,极大多数细菌可浓集在涂点的边缘,呈环圈形分佈。
The direct counting of acid-fast bacteria under the microscope is a basic technique in leprosy experimental study. Currently used methods can be broadly divided into two categories: 1, with a micropipette or calibration of the carrier fluid inoculation loop bacteria will be coated with a diameter of 0.8 ~ 1.13 cm on a glass slide. 2, with the calibration of liquid volume pin needles, dipping bacteria in the slide for spot-like coating, the “needle method.” Although these two methods have their own advantages, they can not solve the problem that droplets concentrate to the center and edge of the coating during the natural drying process, so that the distribution of the bacteria is not uniform. The same coating points in different regions of the amount of bacteria up to 10 times more than the suspension if the tissue content is low, the great majority of bacteria can be concentrated in the painted edge, was ring-shaped distribution.