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测量了３０８ｎｍ紫外激光诱导肺正常和癌组织各７例、肝正常和癌组织各６例在３１０ｎｍ至５５０ｎｍ波段的自体荧光，目的是根据光谱建立一种将正常组织与癌组织区别开来的方法。结果提示：癌组织与正常组织荧光光谱的主要区别是４６０ｎｍ附近次峰强度不同，从而导致了３４０ｎｍ处荧光强度与４４０ｎｍ处荧光强度比（Ｉ３４０／Ｉ４４０）不同。肺正常和癌组织平均Ｉ３４０／Ｉ４４０值分别为１．３０４±０．１９５和２．１２４±０．４１２（Ｐ＜０．０１）；肝正常和癌组织平均Ｉ３４０／Ｉ４４０值分别为２．３６３±０．６７０和１．６７５±０．２２０（Ｐ＜０．０５）。根据癌组织与正常组织Ｉ３４０／Ｉ４４０值差异，可把癌组织与正常组织区分出来，为早期诊断恶性肿瘤提供了一种新的方法。
The 308-nm UV laser-induced autofluorescence of normal and cancerous lung tissue in 7 cases, normal liver tissue, and 6 cases of cancer tissue in the 310-550 nm band was measured in order to establish a method for distinguishing normal tissues from cancer tissues based on spectra. . The results suggest that the main difference between the fluorescence spectra of cancer tissues and normal tissues is that the intensity of the secondary peaks near 460 nm is different, resulting in a difference between the fluorescence intensity at 340 nm and the fluorescence intensity ratio at 440 nm (I340/I440). The average I340/I440 values of normal lung tissue and cancer tissue were 1.304±0.195 and 2.124±0.412, respectively (P<0.01). The average I340/I440 values of normal liver tissue and cancer tissue were 2.363, respectively. ± 0.670 and 1.675 ± 0.220 (P < 0.05). According to the difference between the I340/I440 values of cancer tissues and normal tissues, cancer tissues can be distinguished from normal tissues, providing a new method for early diagnosis of malignant tumors.