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目的建立牛、兔、人晶状体上皮细胞的体外培养，进一步认识晶状体上皮细胞生长、分化规律。方法应用组织块培养方法进行３种晶状体上皮细胞的体外培养，经Ｇｉｍｓａ染色，在倒置显微镜下对培养的晶状体上皮细胞的生长、分化规律进行观察。结果培养至６ｗｋ的人胎儿晶状体上皮细胞中有特征性的“晶状体小体”形成；牛、兔晶状体上皮细胞去分化是发生在第３代，而人晶状体上皮细胞在第４代开始出现去分化；它们传代至第８代时生长都趋于停止，出现老化表现；来自人的晶状体上皮细胞生长增殖率与年龄呈负相关关系（ｒ＝－０．９９６）。结论“晶状体小体”的形成可作为确定晶状体上皮细胞株的一项特征性依据，而体外培养的人、牛、兔晶状体上皮细胞具有相同的有限生长潜能，在相同的条件下，牛、兔晶状体上皮细胞的生长增殖速度比人晶状体上皮细胞快，但易于发生去分化；此外，人晶状体上皮细胞的生长增殖率与年龄密切相关，年龄越小，晶状体上皮细胞的生长增殖速度越快。
Objective To establish the in vitro culture of bovine, rabbit and human lens epithelial cells and to further understand the law of lens epithelial cell growth and differentiation. Methods Three kinds of lens epithelial cells were cultured in vitro by tissue culture method. The growth and differentiation of cultured lens epithelial cells were observed under inverted microscope with Gimsa staining. RESULTS: Human fetal lens epithelial cells cultured to 6wk were characterized by the formation of “lens bodies”. The dedifferentiation of bovine and rabbit lens epithelial cells took place in the third generation, whereas the degeneration of human lens epithelial cells began at the 4th passage ; When they were passaged to the 8th generation, their growth tended to cease and showed the appearance of aging; the growth rate of human lens epithelial cells was negatively correlated with the age (r = -0.996). Conclusion The formation of “lens corpuscle” can be used as a characteristic basis for the determination of lens epithelial cell lines. However, the cultured human, bovine and rabbit lens epithelial cells have the same limited growth potential. Under the same conditions, The growth rate of lens epithelial cells is faster than that of human lens epithelial cells, but it is prone to dedifferentiation. In addition, the growth rate of human lens epithelial cells is closely related with age. The younger the lens epithelial cells, the faster the growth of lens epithelial cells.