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本文通过观察冷应激时mir-210在大鼠肝脏中的表达情况,预测了mir-210在冷应激中的功能。本实验采用了30只12周龄SPF级Wistar雄性大鼠,体重(340±20)g,将大鼠在常温预饲一周后,随机分成急性冷应激组[温度(4±0.1)°C]和常温对照组[温度(24±0.1)°C],急性应激12 h后,提取肝脏,q RT-PCR检测mir-210表达。结果表明,急性冷应激组大鼠肝脏中的mir-210表达量升高,与常温对照组相比差异极显著(P<0.01)。通过生物信息学方法分析发现,mir-210的靶基因有上百种,其中与冷应激相关性较高的靶基因是E2F3、RAD52、ISCU和Ephrin-A3。ISCU能够调节细胞的呼吸代谢,而Ephrin-A3对细胞的增殖和凋亡有显著影响;mir-210的上调还能够直接影响DNA的修复机制,从而导致遗传不稳定。结果提示,冷应激时肝脏mir-210的上调能够对细胞生长发育、能量代谢和遗传等方面产生影响。
In this paper, the expression of mir-210 in rat liver during cold stress was observed to predict the function of mir-210 in cold stress. In this experiment, 30 Wistar male rats of 12 weeks old SPF grade were weighed (340 ± 20) g. Rats were pre-stressed for one week at normal temperature and then randomly divided into acute cold stress group [temperature (4 ± 0.1) ° C ] And normal temperature control group (24 ± 0.1 ° C). After acute stress for 12 h, the liver was harvested and the expression of mir-210 was detected by q RT-PCR. The results showed that the expression of mir-210 in the liver of acute cold stress group increased significantly compared with the normal temperature control group (P <0.01). Bioinformatics analysis showed that there are hundreds of target genes of mir-210, of which target genes related to cold stress are E2F3, RAD52, ISCU and Ephrin-A3. ISCU can regulate the respiratory metabolism of cells, while Ephrin-A3 has a significant effect on cell proliferation and apoptosis; up-regulation of mir-210 can also directly affect DNA repair mechanism, resulting in genetic instability. The results suggest that the upregulation of liver mir-210 in cold stress can affect cell growth and development, energy metabolism and heredity.