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目的:探讨中药复方咳喘康对博莱霉素致肺纤维化大鼠的干预作用及其机理。方法:将60只SD大鼠随机分为正常组、模型组、咳喘康组和激素组。除正常组外,其余3组用博莱霉素A5经气管注入大鼠制作肺纤维化模型,各组于造模后第1天给予相应药物,分别于第7、14、28天分批处死各组大鼠,每次每组5只,比较各组的肺系数、肺组织病理学变化、ELISA法测定肺组织匀浆中TGF-β1含量、RT-PCR法检测TGF-1βmRNA的表达。结果:咳喘康组大鼠肺组织病理学改变较模型组改善,其肺系数、肺组织匀浆中TGF-β1含量、TGF-1βmRNA的表达水平与模型组对照降低,且有随给药天数增加而降低的趋势(P<0.01)。结论:咳喘康可以有效的降低TGF-β1含量及抑制TGF-1βmRNA的表达从而起到拮抗肺纤维化的作用。
OBJECTIVE: To investigate the intervention effect of Kechuankang, a traditional Chinese medicine compound, on bleomycin-induced pulmonary fibrosis in rats and its mechanism. Methods: 60 SD rats were randomly divided into normal group, model group, Kechuankang group and hormone group. In addition to the normal group, the other three groups were treated with bleomycin A5 via the trachea to infuse a rat model of pulmonary fibrosis. Each group was given the corresponding drug on the first day after the model was established. They were sacrificed in batches on the 7th, 14th, and 28th days respectively. Five rats in each group were used in each group. The lung coefficient, pathological changes in lung tissue, TGF-β1 content in lung homogenate were measured by ELISA, and the expression of TGF-β mRNA was detected by RT-PCR. Results: The pathological changes of the lungs in the Kechuankang group were better than those in the model group. The lung coefficient, the content of TGF-β1 in the lung homogenate, and the expression level of TGF-1βmRNA were lower in the model group than in the model group, and there were days after administration. Increased and decreased trend (P<0.01). Conclusion: Kechuankang can effectively reduce the content of TGF-β1 and inhibit the expression of TGF-β mRNA, which plays an important role in antagonizing pulmonary fibrosis.