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目的 观察四君子汤对脾虚患者血浆细胞因子的影响 ,探讨脾虚与细胞因子的关系 .方法 6 6例脾虚患者分为四君子汤治疗组和六味地黄汤治疗组 ,采用免疫分析技术对脾虚患者及其血浆 IL - 2 ,IL - 2 R,IL - 4,IL - 5 ,TNF-α,Ig E,s ICAM- 1等细胞因子水平进行测定 ,用逆转录聚合酶链反应技术 (RT- PCR)对外周淋巴细胞 IL- 4m RNA,IL- 5 m RNA表达进行定量分析 ,用斑点杂交法检测外周血 CD2 m RNA.结果 脾虚患者 IL- 2 ,IL- 4,IL- 5 ,TNF- α,Ig E水平下降 ,s ICAM- 1和 s IL- 2 R水平升高 ,外周淋巴细胞 IL- 4m RNA,IL - 5 m RNA和 CD2 m RNA弱表达 ,表现为免疫功能下降 ,经过四君子汤治疗 ,上述各项指标恢复正常 .结论 四君子汤可通过益气健脾调节细胞因子的水平和功能 ,从而达到增强免疫功能的作用
Objective To observe the effect of Sijunzi decoction on plasma cytokines in patients with spleen deficiency and explore the relationship between spleen deficiency and cytokines. Methods Sixty-six patients with spleen deficiency were divided into Sijunzi Decoction group and Liuwei Dihuang Decoction group. Immunological analysis was used to detect spleen deficiency patients and their plasma levels. IL-2, IL-2 R, IL-4, IL-5, TNF-α, Ig E, s ICAM-1 and other cytokines were measured by reverse transcriptase polymerase chain reaction (RT-PCR). The expression of IL-4 mRNA and IL-5 mRNA in lymphocytes was quantitatively analyzed. Dot-blot hybridization was used to detect CD2 m RNA in peripheral blood. Results IL-2,IL-4,IL-5,TNF-α,Ig E levels in patients with spleen deficiency Decreased, sICAM-1 and sIL-2R levels were elevated, IL-4 mRNA, IL-5 mRNA, and CD2 m RNA were weakly expressed in peripheral lymphocytes, showing decreased immune function. After treatment with Sijunzi Decoction, the above items were The indicators returned to normal. Conclusion Sijunzi Decoction can regulate the level and function of cytokines by replenishing Qi and strengthening the spleen, so as to achieve the effect of enhancing immune function.