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目的研制含氟毒物的检测芯片,实现氟乙酰胺等含氟毒物的快速电化学检测。方法利用氟离子选择电极制备含氟毒物的检测芯片,对芯片各项性能进行测试;建立氟乙酰胺染毒动物模型,使用制备的氟离子芯片检测染毒致死大白兔体内氟离子浓度。结果血液添加标准检测氟离子其线性响应范围为1～10-6mol/L,相关系数为0.9994,平均斜率接近理论斜率,检测限为5×10-7mol/L。染毒组大耳白兔血中氟离子含量较对照组高5～6倍(P<0.01),肝、肾、脑组织中氟离子含量较对照组高2～4倍(P<0.01)。结论此含氟毒物的检测芯片检测氟离子快速、简便、灵敏,可应用于氟乙酰胺等含氟毒物中毒案件的初筛。
Objective To develop a fluorine-containing poison detection chip to achieve rapid electrochemical detection of fluorine-containing toxicants such as fluoroacetamide. Methods Fluoride ion-selective electrode was used to prepare the detection chip containing fluorine-containing poison, and the performance of the chip was tested. Fluoroacetamide-exposed animal model was established. Fluoride ion chip was used to detect the concentration of fluoride ion in the lethally infected rabbit. Results The linear response range of fluoride ion was 1 ~ 10-6mol / L and the correlation coefficient was 0.9994. The average slope was close to the theoretical slope, and the detection limit was 5 × 10-7mol / L. The content of fluoride in the blood of the rabbits in the exposure group was 5-6 times higher than that in the control group (P <0.01). The content of fluoride in the liver, kidney and brain tissues was 2-4 times higher than that in the control group (P <0.01). Conclusion This fluorine-containing poison detection chip detects fluoride ion quickly, easily and sensitively and can be applied to the screening of fluoride poisoning cases such as fluoroacetamide.