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电脉冲因其微创,靶向性强,无明显禁忌而逐渐成为肿瘤治疗研究的热点。其对肿瘤治疗的有效性研究已取得一系列肯定的结果,但其治疗机制仍旧没有得到完全的研究证实。为明确陡脉冲对肿瘤的治疗机制,本实验采用能量可控陡脉冲(Energy controllable steep pulse,ECSP)治疗荷瘤裸鼠,检测既是凝血系统重要启动因子、又是肿瘤浸润和转移指标——组织因子(Tissue factor,TF)在ECSP治疗前后的变化,分析ECSP,肿瘤凝血状态及肿瘤浸润、转移之间的相互作用,浅析ECSP对肿瘤的治疗机理。实验中将裸鼠卵巢癌皮下移植瘤完全随机分为治疗组和未治疗组,ECSP对治疗组作用后,光镜从形态学观察血栓形成,免疫组织化学SP法、RT-PCR对TF进行定位和定量检测。结果显示,ECSP作用后肿瘤血栓明显形成,TF在肿瘤治疗组表达明显降低。结果表明,ECSP促血栓形成,消耗TF,改变肿瘤组织高凝状态;同时TF在卵巢癌组织表达减少,提示ECSP与卵巢癌的侵袭和转移受抑制有关。
Because of its minimally invasive electric pulse, targeted, no obvious taboo and gradually become a hot topic in the treatment of cancer. The effectiveness of cancer treatment has made a series of positive results, but the treatment mechanism has not been fully confirmed. In order to clarify the therapeutic mechanism of steep pulse on tumor, we used Energy-controlledlable steep pulse (ECSP) to treat tumor-bearing nude mice, which is an important factor of coagulation system and an indicator of tumor invasion and metastasis-tissue (Tissue factor, TF) changes in ECSP before and after treatment, analysis ECSP, tumor coagulation status and tumor invasion and metastasis of the interaction between the ECSP analysis of the mechanism of treatment of the tumor. In the experiment, subcutaneously transplanted tumor of ovarian cancer in nude mice were randomly divided into treatment group and untreated group. The effect of ECSP on the treatment group was observed by light microscopy thrombus formation, immunohistochemical SP method and RT-PCR And quantitative detection. The results showed that the tumor thrombus formed obviously after ECSP treatment, and the expression of TF in the tumor treatment group was significantly decreased. The results showed that ECSP thrombosis, consumption of TF, changing the hypercoagulability of tumor tissue; while TF decreased expression in ovarian cancer, ECSP and ovarian cancer invasion and metastasis were inhibited.