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目的:观察黄连和小檗碱对大鼠红细胞氧化性溶血及其抗氧化系统的影响。方法:采用乙酰苯肼(APH)诱导大鼠红细胞氧化性溶血模型,以伯氨喹啉为对照药,观察不同浓度的黄连和小檗碱对血浆游离血红蛋白、血清间接胆红素、全血网织红细胞以及红细胞内抗氧化系统相关酶和蛋白的影响。结果:150 mg.kg-1 APH能引起正常大鼠血浆游离血红蛋白和血清间接胆红素含量及外周血网织红细胞数量显著升高,红细胞6-磷酸葡萄糖脱氢酶(G6PD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)和还原型谷胱甘肽(GSH)含量明显降低;15 mg.kg-1伯氨喹啉能明显加重APH所引起的红细胞氧化性溶血;67～600 mg.kg-1黄连和10.8～97.5 mg.kg-1小檗碱对APH所引起的红细胞氧化性溶血没有明显影响,但600 mg.kg-1黄连和97.5 mg.kg-1小檗碱均能显著增强溶血大鼠红细胞G6PD活力并提高GSH含量,前者尚能增强GSH-Px活力。结论:黄连和小檗碱对APH诱导的大鼠红细胞氧化性溶血没有明显影响。稍高剂量的黄连和小檗碱能通过阻碍APH对G6PD的干扰、维护GSH-Px正常活力和增加GSH含量而发挥一定的红细胞抗氧化保护作用。
Objective: To observe the effects of berberine and berberine on oxidative hemolysis and its antioxidant system in rats. Methods: Oxidative hemolytic model of rat erythrocytes was induced by acetophenone hydrazine (APH). The primary metabolites of berberine were used as control. The effects of different concentrations of berberine and berberine on plasma free hemoglobin, serum indirect bilirubin, Reticulocytes and red blood cells within the antioxidant enzymes and proteins. Results: 150 mg · kg-1 APH could cause plasma free hemoglobin and serum indirect bilirubin levels and the number of peripheral reticulocyte in normal rats significantly increased. The levels of G6PD and glutathione The content of GSH-Px, CAT and SOD and GSH decreased significantly. The contents of GSH-Px, The oxidative hemolytic activity of erythrocytes induced by APH was significantly aggravated by 67μg / kg of rhizoma coptidis and berberine of 10.8-97.5 mg.kg-1, but no significant difference was observed between the two groups .kg-1 Coptis and 97.5 mg.kg-1 berberine can significantly enhance the hemolytic rat erythrocyte G6PD activity and increase GSH content, the former still can enhance GSH-Px activity. CONCLUSION: Coptis and berberine have no obvious effect on APH-induced oxidative hemolysis in rat erythrocytes. Slightly higher doses of berberine and berberine can play a certain role of red blood cell antioxidant protection by hindering the interference of APH on G6PD, maintaining normal GSH-Px activity and increasing GSH content.