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据《生命工学》1986年第1期报道,日本住友化学工业株式会社建立了一种用基因重组方法生产苏云金杆菌制剂(BT剂)的新技术。该社试制了对甘蓝夜蛾有效的BT剂第1号,在此之前生产的BT剂对这种蔬菜害虫是没有杀虫效果的。BT剂原来的生产周期将近为1星期,而现在的新生产方法培养时间缩短为半天,而且,杀虫性蛋白质的活性也提高了将近两倍。通过使用大肠杆菌进行基因重组的方法,获得超过10万的高分子物质,这在世界上也是罕见的。
According to “Life Science”, 1986, No. 1 report, Japan Sumitomo Chemical Co., Ltd. established a new method of gene recombination production Bacillus thuringiensis preparation (BT agent). The agency trial-produced BT agent No. 1, effective against Brassica campestris, to which the BT agent produced had no insecticidal effect. The original BT production cycle was nearly a week old, while the current production method of new production method was shortened to half a day, and the activity of insecticidal protein was almost doubled. By using E. coli for genetic recombination, more than 100,000 macromolecular substances are obtained, which is also rare in the world.