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目的 较全面地观察胃癌患者的细胞免疫功能 ,为胃癌的预测、诊断、病情估计及免疫治疗提供依据。方法 应用酶联免疫双抗夹心法 (ELISA)及碱性磷酸酶抗碱性磷酸酶 (APAAP)桥联酶标法 ,检测 3 4例胃癌患者与对照者血清可溶性白细胞介素 -2受体 (sIL -2R)、T淋巴细胞亚群及自然杀伤 (NK)细胞。结果 胃癌患者sIL-2R水平明显高于对照组 (P <0 0 1) ,Ⅲ、Ⅳ期高于Ⅰ、Ⅱ期 (P <0 0 1) ;CD4、CD8、CD4/CD8低于对照组 (P <0 .0 5 ) ,Ⅲ、Ⅳ期低于Ⅰ、Ⅱ期 (P<0 0 5 ) ;NK细胞百分比显著低于对照组 (P <0 0 1) ,Ⅲ、Ⅳ期明显低于Ⅰ、Ⅱ期 (P <0 0 1)。结论 多指标检测表明胃癌病人细胞免疫功能明显下降。推测细胞免疫功能下降、免疫监视功能低下可能是患胃癌的重要原因 ;检测细胞免疫功能对胃癌的预测、诊断、病情估计及免疫治疗的选择均有重要价值。
Objective To observe the cellular immune function of gastric cancer patients more comprehensively, and to provide basis for the prediction, diagnosis, assessment of disease status and immunotherapy of gastric cancer. Methods Serum soluble interleukin-2 receptors were detected in 34 patients with gastric cancer and controls using enzyme-linked immunosorbent assay (ELISA) and alkaline phosphatase anti-alkaline phosphatase (APAAP) bridging enzyme labeling method. sIL-2R), T lymphocyte subsets, and natural killer (NK) cells. Results The level of sIL-2R in gastric cancer patients was significantly higher than that in control group (P <0 01), and it was higher in stage III and IV than in stage I and II (P <0 01). The levels of CD4, CD8 and CD4/CD8 were lower than those in the control group ( P <0.05), stage III and IV were lower than stage I and II (P <0 05); the percentage of NK cells was significantly lower than that of the control group (P <0 01), stage III and IV were significantly lower than I. Stage II (P < 0 01). Conclusion Multi-indicator detection showed that the cellular immune function of gastric cancer patients decreased significantly. It is speculated that the decline of cellular immune function and immune surveillance may be an important cause of gastric cancer; detection of cellular immune function has important value in the prediction, diagnosis, assessment of disease status and selection of immunotherapy for gastric cancer.