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目的观察有机磷中毒(AOPP)小鼠肝脏、脾脏SOCS-1、SOCS-3及TNF-amRNA的表达,探讨其在AOPP致多器官功能障碍综合症(MODS)中的发病机制,以期为MODS的防治提供新的策略。方法健康小鼠随机分成敌敌畏组、生理盐水对照组和正常对照组,分别于染毒后2、6、12、24h取肝脾组织,以逆转录聚合酶链反应(RT-PCR)测定SOCS-1、SOCS-3、TNF-a表达水平。结果AOPP小鼠肝脾SOCS-1、SOCS-3、TNF-a表达均升高,与对照组比较差异有统计学意义(P均<0.05)。结论AOPP可导致细胞因子TNF-a失调,可能参与了AOPP后SIRS向MODS的发病过程;若能抑制炎症反应的失控,则对中毒MODS的治疗将有新的突破。
Objective To investigate the expression of SOCS-1, SOCS-3 and TNF-amRNA in liver and spleen of mice with organophosphate poisoning (AOPP) and to explore their pathogenesis in MODS caused by AOPP, Prevention provides new strategies. Methods Healthy mice were randomly divided into three groups: the dichlorvos group, the saline control group and the normal control group. The liver and spleen tissues were harvested at 2, 6, 12 and 24 hours after the treatment, respectively. SOCS- 1, SOCS-3, TNF-a expression levels. Results The expressions of SOCS-1, SOCS-3 and TNF-a in liver and spleen in AOPP mice were significantly increased compared with those in control group (all P <0.05). CONCLUSION: AOPP can induce the imbalance of cytokine TNF-a, and may be involved in the pathogenesis of SIRS to MODS after AOPP. If it can inhibit the out-of-control of inflammatory reaction, new breakthroughs will be made in the treatment of poisoning MODS.