在小鼠骨髓细胞体外微量液体培养中,通过观察DNA合成时~3H-TdR掺入量的变化,来测定粒单系祖细胞的增殖状态。实验结果表明:此方法具有培养周期短,操作较简单,节省培养材料和被测样品量少等优点。在与体外半固体琼脂CFU-GM培养法相配合中,能正确反应出被测样品对粒单系祖细胞的增殖状态和增殖能力。 Proliferation status of granulocyte-monocyte progenitor cells was determined by observing changes in the amount of 3H-TdR incorporation during DNA micro-liquid culture of mouse bone marrow cells in vitro. The experimental results show that this method has the advantages of short cultivation period, simple operation, less material and less sample. In combination with the in vitro semi-solid agar CFU-GM culture method, the proliferative state and proliferation ability of the test sample on the monocyte-derived progenitor cells can be accurately reflected.