用套式PCR(nPCR)对 3 5例肝硬化患者血清HBV前C区和基本核心基因启动子 (BCP)进行扩增 ,阳性者用直接测序法进行序列分析。结果 2 3例HBVDNA阳性 ,阳性率为 65 7% ( 2 3 / 3 5 ) ,无正常序列标本 ,与e抗原产生有关的突变 :nt1762、1764双突变 (AT、GA) ,占 73 9% ( 17/ 2 3 ) ;nt1896点突变 (GA) ,导致终止密码产生 ,占2 1 7% ( 5 / 2 3 ) ;nt185 8点突变 (TC) ,占 2 1 7% ,其中标本 417、42 6、43 0同时在nt 185 6发生点突变 (CT)。其它的突变有nt1799点突变 (CG) ,占 47 8% ( 11/ 2 3 ) ,为无义突变 ;有 6例在nt1846发生点突变AT ,4例nt180 2 - 180 4发生突变 (TTCCGT) ;4例nt175 2位点突变 (AG) ;标本 43 2在nt175 1插入TG导致移码突变 ,并在nt1774- 1874发生缺失突变。说明HBVBCP和前C突变株在肝硬化患者中很常见 ,提示这些突变与肝硬化的发生有关 Envelope PCR (nPCR) was used to amplify the serum pre-HBV precore and basic core promoter (BCP) in 35 cirrhotic patients. Positives were sequenced by direct sequencing. Results The positive rate of HBVDNA was 65 7% (2 3/3 5) in 23 cases with no normal sequence and the mutations associated with e antigen: nt1762 and 1764 double mutations (AT, GA) The mutation of nt1896 (GA) resulted in the termination codon, accounting for 21.7% (5/23) of nt1858 mutations (TC), accounting for 21.7% %, Of which 417,42 6,43 0 were mutated at nt 185 6 simultaneously (CT). Other mutations were nt1799 point mutation (CG), accounting for 47.8% (11/2 3), which were nonsense mutations. Six of them showed point mutations AT at nt1846 and four of nt180 2 - 1804 (TTC CGT); 4 cases of nt175 2 site mutation (A  G); specimen 43 2 inserted TG at nt175 1 resulted in frameshift mutation and deletion mutation at nt1774-1874. Description of HBVBCP and pre-C mutations in patients with cirrhosis is very common, suggesting that these mutations and the occurrence of cirrhosis