分别采用紫外分光光度法测定二氢乳清酸(DHO)的减少量、PDAB显色法测定N-氨甲酰-天冬氨酸(CA-asp)的生成量来测定二氢乳清酸酶酶活,并对两种方法进行了比较。结果表明紫外分光光度法和PDAB显色法均有良好的回收率,分别为99.4%～101.2%、98.0%～101.3%。但由于PDAB显色法的检测范围为0.05～1.00g/L,较紫外法的检测范围0.05～0.30g/L更为宽泛,仪器要求低,便于实际操作。因此,采用PDAB显色法测定二氢乳清酸酶酶活较为方便。 The decrease of dihydrobutyric acid (DHO) was measured by UV spectrophotometry, and the production of N-carbamoyl-aspartic acid (CA-asp) was determined by PDAB colorimetric assay Enzyme activity, and the two methods were compared. The results showed that both UV spectrophotometry and PDAB colorimetric method had good recoveries of 99.4% ~ 101.2% and 98.0% ~ 101.3%, respectively. However, PDBA colorimetric detection range of 0.05 ~ 1.00g / L, UV detection range of 0.05 ~ 0.30g / L is more broad, the instrument requirements are low, easy to operate. Therefore, the use of PDAB colorimetric assay dihydro lactase enzyme activity is more convenient.