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为了解脾交感神经在免疫功能增强时的形态变化，用免疫组织化学方法观察了ＢＡＬＢ／ｃ小鼠脾内生长相关蛋白样免疫反应（ＧＡＰ－４３－ＬＩ）性神经在结核菌素衍生蛋白（ＰＰＤ）诱发的免疫反应高峰期数量和分布的变化。结果显示，动物在接受两次ＰＰＤ注射（２１ｄ＋７ｄ）后，脾ＧＡＰ－４３－ＬＩ神经纤维密度明显增加并伴有分布和形态的变化。于对照动物，脾ＧＡＰ－４３－ＬＩ神经纤维主要分布于血管周围，少量伸入动脉周围淋巴鞘的脾基质中，而在免疫刺激的动物，不仅血管周围的神经纤维明显增多，动脉周围淋巴鞘外层、边缘区和红髓等免疫应答活跃的部位也出现许多神经纤维。神经纤维分支和纤维上膨体明显增多。以上结果提示：免疫应答中脾神经成分发生活跃的结构重塑，这些变化有可能与神经的免疫调节功能有关。
In order to understand the morphological changes of the splenic sympathetic nerve during the enhancement of immune function, the growth-associated protein-like immunoreactivity (GAP-43-LI) of the spleen in BALB / c mice was observed by immunohistochemistry in the presence of tuberculinin- PPD) induced immune response peak number and distribution changes. The results showed that the density of splenic GAP-43-LI nerve fibers was significantly increased and accompanied by the distribution and morphological changes of the animals after receiving PPD twice (21d + 7d). In the control animals, the splenic GAP-43-LI nerve fibers were mainly distributed in the perivascular area and splenomegaly in the splenic matrix of the peripheral lymphatic sheath. However, in the immune-stimulated animals, not only the peripheral nerve fibers were significantly increased, The outer layer, the marginal zone and red pulp and other parts of the immune response is also active in many nerve fibers. Nerve fiber branches and fibrous bulge increased significantly. The above results suggest that there is an active structural remodeling of the splenic nerve components in the immune response, which may be related to the immunomodulatory function of the nerve.