论文部分内容阅读
目的：探讨原位ＰＣＲ技术在ＨＢＶ低水平感染中的意义。方法：用原位ＰＣＲ方法检测４２例肝炎患者石蜡包埋肝组织的ＨＢＶ－ＤＮＡ并与原位杂交技术检测结果进行比较。结果：原位ＰＣＲ检测ＨＢＶ－ＤＮＡ的检出率为５０％，明显高于原位杂交法（１９％，Ｐ＜０．０１），肝组织中ＨＢＶ－ＤＮＡ检出率与临床诊断无关。阳性杂交信号均位于肝细胞浆，呈簇状和弥散状分布。结论：原位ＰＣＲ检测肝组织ＨＢＶ－ＤＮＡ具有快速、灵敏、特异的优点，可检出ＨＢＶ低水平感染，适用于ＨＢＶ－ＤＮＡ的组织细胞定位研究，部分ＨＢｓＡｇ阴性或抗ＨＢｓ阳性患者仍存在ＨＢＶ低水平复制，ＨＢＶ感染是部分ＨＢＶＭ阴性患者的病因。
Objective: To investigate the significance of in situ PCR in low-level HBV infection. Methods: HBV-DNA of paraffin-embedded liver tissues of 42 patients with hepatitis were detected by in situ PCR and compared with the results of in situ hybridization. Results: The detection rate of HBV DNA by in situ PCR was 50%, which was significantly higher than that of in situ hybridization (19%, P <0.01). The detection rate of HBV-DNA in liver tissue was not related to clinical diagnosis. Positive hybridization signals are located in the liver cytoplasm, clustered and diffuse distribution. Conclusion: Detection of HBV-DNA in liver tissue by in situ PCR is rapid, sensitive and specific. It can detect low-level HBV infection and is suitable for tissue-cell localization research of HBV-DNA. Some HBsAg-negative or anti-HBs-positive patients still have HBV Low-level replication, HBV infection is part of the cause of HBVM-negative patients.