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目的：探讨肝癌患者肝组织中 ＨＢｘＡｇ与 Ｆａｓ／ＦａｓＬ的表达状况及血清中 ＨＢＶ Ｘ基因与 Ｆａｓ／ＦａｓＬ的水平。方法：用ＳＰ法对３４例肝癌组织ＨＢｘＡｇ、Ｆａｓ与ＦａｓＬ表达状况进行分析，用ＥＬＩＳＡ法对３０例肝癌、３２例肝硬化及２０例正常人血清ｓＦａｓ与 ｓＦａｓＬ含量进行分析，并用ＰＣＲ法对肝癌与肝硬化患者血清ＨＢＶ Ｘ基因表达进行检测。结果：ＨＢｘＡｇ、Ｆａｓ与ＦａｓＬ在肝癌表达的阳性率分别为９７．０６％、８５．２９％及１００％。阳性信号均主要位于胞浆，且三者可在同一癌组织的相同区域出现阳性染色，Ｒｅｄｉｔ分析示三者的阳性程度无统计学差异（Ｐ＞０．０５）。血清ｓＦａｓ在肝癌、肝硬化与正常人中的含量分别为（７２２．９７±３２１．１３）μｇ／Ｌ、（８０１．９０±４１９．９４）μｇ／Ｌ和（２２４．０７±１４８．２３）μｇ／Ｌ，其中肝癌与肝硬化组显著高于正常人（Ｐ＜ ０． ０１）；血清 ｓＦａｓＬ在肝癌、肝硬化与正常人中的含量分别为（１５２． ２７±７． ９９）μｇ／Ｌ、（ １６２． ９７±１２． ４０）μｇ／Ｌ和（１５４．９９± ６．９６）μｇ／Ｌ，其中肝硬化组含量高于肝癌与正常人（ Ｐ＜０．０１）。血清ＨＢＶ Ｘ基
Objective: To investigate the expression of HBxAg and Fas/FasL in the liver tissue of patients with hepatocellular carcinoma and the levels of HBV X gene and Fas/FasL in serum. Methods: The expression of HBxAg, Fas and FasL in 34 cases of hepatocellular carcinoma were analyzed by SP method. The levels of serum sFas and sFasL in 30 cases of hepatocellular carcinoma, 32 cases of cirrhosis and 20 normal persons were analyzed by ELISA method. Serum HBV X gene expression was detected in patients with cirrhosis. Results: The positive rates of HBxAg, Fas and FasL in hepatocellular carcinoma were 97.06%, 85.29% and 100%, respectively. The positive signals were mainly located in the cytoplasm, and the positive staining in the same area of the same cancer tissue was found in all three cases. The Redit analysis showed that there was no significant difference in the positive degree (P>0.05). The levels of serum sFas in liver cancer, liver cirrhosis and healthy persons were (722.97±321.13) μg/L, (801.90±419.94) μg/L, and (224.07±148.23), respectively. Μg/L, which was significantly higher in liver cancer and liver cirrhosis group than normal (P < 0. 01); serum sFasL in liver cancer, cirrhosis and normal people were (152.27 ± 7.99) μg/L , (162.97±12.40)μg/L and (154.99±6.96)μg/L, the content of cirrhosis group was higher than that of liver cancer and normal people (P<0.01). Serum HBV X-base