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水稻低氮相关性状的遗传分析对研究氮素吸收和利用效率及培养耐性品种具有重要意义。缺氮是限制作物生长和产量提高的重要因素。利用一套以9311为遗传背景携带日本晴置换片段的染色体片段置换系为材料,对低氮胁迫下水稻苗期性状进行了QTL定位。共定位到高氮和低氮下株高、主根长、根干质量、苗干质量和总干质量及其相对性状的44个QTLs,其中2种氮水平下检测到28个QTLs,低氮与高氮相对性状检测到16个QTLs。检测到2个QTL热点区,聚集了不同氮水平及其相对性状的多个QTLs,通过搜索预测基因,发现这2个区域包含氮代谢的关键基因,说明极有可能是氮吸收和利用相关基因控制了这些QTLs的表达。以上结果将为利用分子标记辅助选育耐低氮品种提供依据。
Genetic analysis of low nitrogen-related traits in rice is of great importance for studying nitrogen absorption and utilization efficiency and cultivating tolerant cultivars. Nitrogen deficiency is an important factor that limits crop growth and yield. A QTL mapping of rice seedling traits under low nitrogen stress was carried out using a chromosomal segment substitution line carrying the Nipponbare fragment with 9311 as the genetic background. A total of 44 QTLs were mapped to plant height, main root length, root dry weight, seedling dry weight, total dry weight and their relative traits under high and low nitrogen conditions. Twenty-eight QTLs were detected under two nitrogen levels, Six QTLs were detected in high nitrogen relative traits. Two QTLs were detected and clustered with multiple QTLs with different nitrogen levels and their relative traits. By searching the predicted genes, it was found that these two regions contained key nitrogen metabolism genes, which indicated that it was most likely nitrogen related genes Controlled the expression of these QTLs. The above results will provide the basis for molecular marker-assisted breeding of low-nitrogen-tolerant varieties.