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目的 探索一种适合于老龄大鼠脑梗死研究的大脑中动脉闭塞 (MCAO)模型。方法 用神经功能缺损评分、墨汁或 TTC染色、病理学观察以及脑组织含水量测定等方法比较研究老龄大鼠线栓与改良自体血栓 MCAO模型的稳定性。结果 血栓组与线栓组成功率分别为 95.83%和 1 5% ,前者神经功能缺损较后者重 (评分为 8.59± 3.52与 1 4 .0 6± 4.0 4 ,P=0 .0 0 1 ) ,前者梗死体积亦高于后者 (2 60 .67± 1 4 .65mm3与 1 51 .0 0±98.1 4 mm3 ,P<0 .0 5) ;脑组织含水量血栓组明显高于线栓组 (P=0 .0 0 1 )。后者蛛网膜下腔出血的发生率为 2 %。结论 自体血栓性 MCAO模型成功率高、梗死范围恒定、可重复性好 ,较线栓更适合于老龄大鼠局灶性脑缺血的研究。
Objective To explore a model of middle cerebral artery occlusion (MCAO) suitable for the study of cerebral infarction in aged rats. Methods The neurological deficit score, ink or TTC staining, pathological observation and determination of brain water content were used to compare the stability of MCAO model with suture thrombus and ameliorating thrombosis in aged rats. Results The success rates of thrombosis and thrombus were 95.83% and 15%, respectively. The former had more severe neurological deficits (score 8.59 ± 3.52 vs 14.06 ± 4.04, P = 0.010) The former infarction volume is also higher than the latter (2 60.67 ± 14.56mm3 and 151.098.1 4 mm3, P <0 05); brain water content of thrombosis was significantly higher than the group of thread P = 0 .0 0 1). The latter the incidence of subarachnoid hemorrhage was 2%. Conclusion Autologous thrombosed MCAO model has high success rate, constant infarct range, good repeatability and is more suitable for the study of focal cerebral ischemia in aged rats.