本文以OKT系列单克隆抗体作为检测试剂，采用直接SPA菌体花环法对62例流行性乙型脑炎患者外周血T淋巴细胞及亚群进行了动态检测。结果表明，发病初期OKT8细胞百分比即明显增高，以轻中型为著，于恢复期降至正常水平。重型及极重型OKT4细胞百分比极期降低，其OKT8细胞百分比接近正常水平。OKT4／OKT8比值均下降。表明流行性乙型脑炎的发病过程与免疫状况有一定关系。 In this paper, OKT series of monoclonal antibodies as a detection reagent, the use of direct SPA bacterial rosette method of 62 cases of Japanese encephalitis in patients with peripheral T lymphocytes and subsets were dynamically detected. The results showed that the percentage of OKT8 cells was significantly increased in the early stages of disease, with light to medium size, and returned to normal in the recovery phase. The percentages of heavy and very heavy OKT4 cells decreased extremely and the percentage of OKT8 cells was near normal. OKT4 / OKT8 ratio decreased. It shows that the pathogenesis of Japanese encephalitis and immune status have a certain relationship.