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通过诱变手段可能选育高毒效苏云金杆菌(Eacillus thuringiensis)菌株。用紫外线处理B.thuringiensis(7216)。对于提高对昆虫的致病作用,已有报道。Norris用紫外线照射后产生的突变株,对大菜粉蝶的致病力提高了7倍,这个突变株在处理前就有每个细菌不只产生一个晶体的倾向,处理后则能正常产生多晶体。本文报道,采用紫外线处理(7216),对诱变菌株的产品能力及其对昆虫的致病力,并从中分离到对斜纹夜蛾毒力较强的菌株。材料和方法 (一)、出发菌株:苏云金杆菌(7216),本所分离并保存。生测对照菌株:HD-1,引自湖南省微生物研究所。
Highly toxic Eacillus thuringiensis strains may be selected by mutagenesis. B.thuringiensis (7216) treated with UV light. For improving the pathogenic effect on insects, has been reported. Norris mutants, produced by UV irradiation, showed a sevenfold increase in pathogenicity to the cabbage butterfly. This mutant had a tendency for each bacterium to produce more than one crystal prior to treatment, and polycrystals normally formed after treatment. This paper reports the UV-treated (7216), the mutagenized strains of the product capacity and its virulence to insects, and isolated from strong strains of Spodoptera litura. Materials and methods (A), the starting strain: Bacillus thuringiensis (7216), the separation and preservation. Bioassay control strain: HD-1, from Hunan Institute of Microbiology.