论文部分内容阅读
运用傅里叶变换红外光谱技术研究了正常组织(30例)和细胞株(SW 620),正常细胞核和癌变细胞核的红外光谱特征,为红外光谱诊断结直肠癌奠定了细胞及亚细胞水平的实验基础。将所得光谱图的峰位及峰强比进行统计分析发现:细胞株(SW 620)和癌变细胞核中谱带(2 925,1 240和1 085cm-1)的峰位均向高波数移动(p<0.05),而谱带1 400cm-1的峰位均向低波数移动(p<0.05);细胞株(SW 620)中峰强比(I1 650/I1 460,I1 400/I1 460,I1 240/I1 460,)较正常组织升高(p<0.05),而I1 740/I1 460则降低(p<0.01)。癌变细胞核中(I1 650/I1 460,I1 400/I1 460,I1 240/I1 460)较正常细胞核亦升高(p<0.05).这些差异可作为区别组织良恶性的指标。
Fourier transform infrared spectroscopy was used to study the infrared spectra of normal tissue (SW306) and cell line (SW620), normal nucleus and cancerous nucleus, which laid the foundation for the determination of cellular and subcellular level in the diagnosis of colorectal cancer by FTIR basis. The statistical analysis of the peak position and the peak-to-peak ratio of the obtained spectrum showed that the peak positions of the bands (2,925,1,240 and 1 085cm-1) in the cell line (SW 620) and the cancerous nucleus both shifted to a high wavenumber <0.05), while the peak position at 1 400 cm-1 of the band shifted to a low wave number (p <0.05). The ratio of the peak intensities (I1 650 / I1 460, I1 400 / I1 460, I1 240 / I1 460, compared with normal tissue (p <0.05), while I1 740 / I1 460 decreased (p <0.01). The cancerous nuclei (I1 650 / I1 460, I1 400 / I1 460, I1 240 / I1 460) also increased compared with the normal nucleus (p <0.05) .This difference could be used as an index to distinguish benign and malignant tissues.