论文部分内容阅读
目的:探讨适合中药复方筛选的α-萘异硫氰酸酯(ANIT)急性肝损伤改良造模方法及痰热清注射液的保护作用。方法:首先对ANIT传统造模方法改良;再次复制改良的急性肝损伤模型,首次染毒6h后分为模型组、甘草酸二铵组、腺苷蛋氨酸组、痰热清低(0.469生药/kg)、中(0.9715g生药/kg)、高(1.9095g生药/kg),连续尾静脉给药7天,同时设正常组。观察小鼠一般情况,HE染色观察肝组织炎症病理,试剂盒检测血清肝功能。结果:建立稳定改良的急性肝损伤模型。药效实验中,痰热清注射液0.9715g生药/kg(中剂量)、1.9095g生药/kg(高剂量)显著降低血清生化指标,改善肝组织炎症病理,减少坏死面积、炎细胞浸润及胆管上皮细胞增生。结论:改良造模方法后成功建立适合中药复方筛选的ANIT急性肝损伤模型;痰热清于0.9715g生药/kg(中剂量)、1.9095g生药/kg(高剂量)发挥保肝降酶、利胆退黄的保护作用。
OBJECTIVE: To explore the improved method for modeling acute liver injury induced by α-naphthalene isothiocyanate (ANIT) and the protective effect of Tanreqing Injection suitable for traditional Chinese medicine compound screening. Methods: First, the traditional method of ANIT modeling was improved; once again, the model of acute liver injury was duplicated and divided into model group, diammonium glycyrrhizinate group, adenosyl methionine group, low phlegm-heat level ), Medium (0.9715g crude drug / kg), high (1.9095g crude drug / kg), and continuous tail vein administration for 7 days. The general situation of mice was observed, the pathological changes of liver tissue were observed by HE staining and the serum liver function was detected by kit. Results: Stable and improved acute liver injury model was established. Pharmacodynamics experiment, Tanreqing injection 0.9715g crude drug / kg (medium dose), 1.9095g crude drug / kg (high dose) significantly reduce serum biochemical indicators, improve liver inflammation and pathology, reduce necrosis, inflammatory cell infiltration and bile duct Epithelial cell proliferation. CONCLUSIONS: Acute liver injury model of ANIT which is suitable for the screening of compound Chinese herbs was established successfully after the improved modeling method. Tanreqing was used to protect liver and reduce enzymes in 0.9715g crude drug / kg (medium dose) and 1.9095g crude drug / kg (high dose) Gallback yellow protective effect.