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目的研究鸡Ⅱ型胶原蛋白诱导性关节炎(CIA)小鼠Treg细胞及转录因子Foxp3的动态变化与类风湿关节炎疾病进展的关系。方法 147只DBA1/J小鼠随机分为正常对照组和模型组,制备Ⅱ型胶原乳剂免疫模型组小鼠,于初次免疫后的d7、d14、d21,加强免疫后的d7、d14、d21、d35无菌摘取腹股沟淋巴结提取淋巴细胞,应用流式细胞技术和荧光定量PCR技术检测各组小鼠的Treg细胞及转录因子Foxp3变化情况。结果模型组Treg细胞及转录因子Foxp3在初次免疫d14、d21较正常组有所升高(P<0.05)。模型组转录因子Foxp3表达量在加强免疫d14时高于正常组(P<0.05)。模型组Treg细胞表达在加强免疫d7、d14、d21逐渐上升,加强免疫d21时模型组Treg细胞表达与正常组相比有所增多(P<0.05)。模型组Treg细胞和转录因子Foxp3表达量在加强免疫d35与正常组相比均有所升高(P<0.05,P<0.01)。结论 Treg细胞及其转录因子Foxp3的动态变化可能和RA发展具有一定关系。
Objective To investigate the relationship between the dynamic changes of Treg cells and the transcription factor Foxp3 and the progression of rheumatoid arthritis in chicken collagen type II-induced arthritis (CIA) mice. Methods A total of 147 DBA1 / J mice were randomly divided into normal control group and model group to prepare type Ⅱ collagen emulsion immunized mice. After d7, d14 and d21 after primary immunization, Lymphocytes were extracted by aseptic extraction of inguinal lymph nodes. Flow cytometry and fluorescent quantitative PCR were used to detect the changes of Treg cells and Foxp3 in each group. Results The Treg cells and transcription factor Foxp3 in the model group were higher than those in the normal group on the first immunization (P <0.05). The expression of Foxp3, a transcription factor of model group, was higher than that of normal group on d14 (P <0.05). The expression of Treg cells in the model group increased gradually on d7, d14 and d21, and the expression of Treg cells in the model group increased significantly (P <0.05). Compared with the normal group, the expression of Treg cells and transcription factor Foxp3 in model group increased at d35 (P <0.05, P <0.01). Conclusion The dynamic changes of Treg cells and its transcription factor Foxp3 may be related to the development of RA.