方中达先生曾提出,玻片培养是好的检视法,本文报导一种以“玻片培养”为基础,制作具分生孢子梗和分生孢子排列的青霉属(Penicillium)菌落永久片的方法,现在简报如下: 方法 1.取洁净的玻片,上滴1—2滴适宜的培养液。例如培养青霉菌时,可用桔子的汁。 2.挑取适量菌原接种,注意疏密均匀。 3.适温保湿培养直至菌丝繁茂,形成菌落,分生孢子梗和分生孢子大量生长,菌落边缘是菌丝分布疏密适度之处。 4.火焰固定。酒精灯火焰上来回荡烘至分生孢子梗绝大部份倒伏。 5.龙胆紫滴染1—2分钟。吸水纸仔细吸 Mr. Fang Zhongda has suggested that slide culture is a good method of detection. This article reports a technique based on “slide culture” for the production of permanent Penicillium colonies with conidiophores and conidia arrangement Method, now briefed as follows: Method 1. Take a clean glass slide, drop 1-2 drops of appropriate culture medium. For example, cultivating penicillium, orange juice can be used. 2. Pick the right amount of bacteria inoculated, pay attention to sparse and uniform. 3. Suitable temperature moisturizing culture mycelium lush, the formation of colonies, conidiophores and conidia grow in large numbers, colony edge is mycelium distribution density appropriate. 4 flame fixed. The alcohol lamp flames up and down to the conidiophore most of the lodging. 5 gentian violet drip 1-2 minutes. Absorb the absorbent paper carefully