目的 :探讨血管内皮细胞生长因子 ( VEGF)在肝硬化门静脉高压症中的作用。方法 :对 1 8例肝硬化门静脉高压症大鼠 (分早期、中期、晚期 3期 )和 6例正常大鼠脾脏切除标本 ,行原位杂交法 ,检测 VEGF的 m RNA表达。结果 :门静脉高压组大鼠 VEGF的m RNA高表达 ,其表达率分别为早期 5 0 .0 % ,中期 66.7% ,晚期 1 0 0 %。对照组阳性表达率为 1 6.7%。结论 :1 VEGF是最有力的血管生成因子 ;2 VEGF随肝硬化门脉高压严重程度而阳性表达率有所增加 Objective: To investigate the role of vascular endothelial growth factor (VEGF) in cirrhosis and portal hypertension. Methods: The expression of VEGF mRNA in 18 cirrhotic patients with portal hypertension (divided into early, middle and late stages) and 6 normal spleen specimens were detected by in situ hybridization. Results: The expression of m RNA of VEGF in portal hypertensive rats was high, the expression rates were 50.0% in early stage, 66.7% in middle stage and 100% in late stage, respectively. The positive rate of the control group was 1 6.7%. Conclusions: 1 VEGF is the most potent angiogenic factor; 2 VEGF is increased with the severity of cirrhosis and portal hypertension