采用ＩＦＡＴ法检测特异ＩｇＧ荧光抗体，用ＭＣＰＥＮＴ法检测中和抗体滴度，追踪流行病学防病效果。结果表明，该疫苗的安全性较好，总反应率为３１９％，中强反应１８２％，反应以局部一过性疼痛多见，无异常反应。疫苗３针全程免疫后２周、加强前、加强后２周、１年和２年，３年中和抗体阳转率分别为３１４０％、９３３％、８７５０％、５３８５％、３８１０％和３５００％。荧光抗体阳转率分别为８４９１％、２２５０％、８５００％、１５７９％、１０２０％和９０５％。疫苗的流行病学防病效果好，３针全程免疫后（１９９５４）至今（１９９９．３）接种者无人发病，对照组发病８例，保护率为１００００％，观察到试区疫情较四周非试区低，疫苗复盖率达５０％以上，似可有效控制疫情。发现有１例接种者在经过一个流行高峰期后荧光抗体较前呈４周以上增高，但无临床表现。未发现接种人群中有免疫（感染）增强问题。 IFAT method was used to detect specific IgG fluorescent antibody, neutralizing antibody titer was detected by MCPENT method, and the epidemiological control effect was followed. The results showed that the vaccine was safe, with a total response rate of 3.19% and a moderate to strong response of 1.82%. The reaction was characterized by local transient pain and no abnormal reaction. Three weeks after the vaccine immunization, the positive rate of positive antibody in three years after immunization was 3140%, 933%, 8750%, 533 85%, 38  10% and 35  00%. The positive rate of fluorescent antibody was 8491%, 2250%, 8500%, 1579%, 1020% and 905% respectively. Epidemiology of vaccine prevention effect is good, 3-pin full-course immunization (1995 4) so ​​far (1999.3) vaccination of no one disease, the control group of 8 patients with a protective rate of 10000% observed in the trial area The epidemic is lower than the non-pilot area around the four weeks, covering more than 50% of the vaccine, it seems to be effective in controlling the outbreak. Fluorescent antibodies were found in one of the vaccinees who had increased for more than 4 weeks after a prevalent peak but showed no clinical signs. No increase in immunization (infection) was found in vaccinated populations.