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为了研究海洋真菌Aspergillus sp.SCS-KFD03次级代谢产物的活性成分,采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和高效液相色谱等技术从其发酵液中分离得到7个化合物,运用各种波谱方法鉴定为3β,5α-dihydroxy-(22E,24R)-ergosta-7,22-dien-6-one(1);3β,4α-dihydroxy-26-methoxyergosta-7,24(28)-dien-6-one(2);麦角甾醇(22E,24R)-ergosta-5,7,22-trien-3β-ol(3);leporin B(4);astellolide G(5);astellolide B(6)和astellolide A(7),并测定化合物的抗氧化活性、乙酰胆碱酯酶和α-糖苷酶抑制活性。结果表明:化合物2,3,5和6具有一定的抗氧化活性,化合物2具有较弱的乙酰胆碱酯酶抑制活性,化合物4具有强于阳性药阿卡波糖的α-糖苷酶抑制活性。
In order to study the active constituents of secondary metabolites of marine fungus Aspergillus sp. SCS-KFD03, seven compounds were isolated from their fermentation broth by silica gel column chromatography, Sephadex LH-20 gel column chromatography and high performance liquid chromatography Various spectroscopic methods identified 3β, 5α-dihydroxy- (22E, 24R) -ergosta-7,22-dien-6-one (1); 3β, 4α-dihydroxy- 26-methoxyergosta-7,24 dien-6-one (2); ergosterol (22E, 24R) -ergosta-5,7,22-trien-3β- ol (3); leporin B (4); astellolide G ) And astellolide A (7), and the compounds were tested for their antioxidant activity, acetylcholinesterase and alpha-glucosidase inhibitory activity. The results showed that compounds 2, 3, 5 and 6 had some antioxidant activity, compound 2 had weaker acetylcholinesterase inhibitory activity, and compound 4 had stronger α-glucosidase inhibitory activity than that of the active drug acarbose.