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目的研究凝固酶阴性葡萄球菌(CNS)抗药基因携带及其对氯己定类消毒剂抗力变化情况。方法采用细菌分离鉴定方法和基因扩增技术,对医院环境和医务人员手上采样标本进行了分离培养和检测。结果在841份标本中,检出63株CNS,检出率为7.49%。63株CNS中,有12株检出qacA基因,检出率为19.05%。醋酸氯己定对12株CNS最小抑菌浓度范围在0.24 mg/L～1.96 mg/L,其中有7株CNS的最小抑菌浓度高于标准菌株者;最小杀菌浓度范围在7.81 mg/L～500 mg/L,其中有9株CNS的最小杀菌浓度明显高于标准菌株。结论医院环境表面和医务人员手上CNS检出率较高,多数携带qacA基因的菌株对醋酸氯己定消毒剂抗力有增加的趋势。
Objective To study the resistance of coagulase-negative staphylococci (CNS) carriers and their resistance to chlorhexidine disinfectants. Methods Bacterial isolation and identification methods and gene amplification techniques were used to isolate, culture and test samples collected from hands of hospital environment and medical personnel. Results Among 841 specimens, 63 CNS were detected with a detection rate of 7.49%. Among 63 CNS strains, 12 strains detected qacA gene with a detection rate of 19.05%. The minimum inhibitory concentrations of chlorhexidine acetate against 12 CNS strains ranged from 0.24 mg / L to 1.96 mg / L, of which 7 were the lowest. The minimum bactericidal concentrations ranged from 7.81 mg / L ~ 500 mg / L. Among them, the minimum bactericidal concentration of 9 CNS strains was significantly higher than that of the standard strains. Conclusion The detection rate of CNS in the surface of hospital environment and medical staff is high. Most strains carrying qacA gene have a tendency of increasing the resistance to chlorhexidine acetate disinfectant.