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目的对树鼩携带的可培养微生物群系进行初步调研,为制定树鼩的微生物学等级标准提供依据。方法采集61只野生树鼩呼吸道分泌物、肠道内容物和体表毛发,接种于多种培养基进行细菌分离培养,并进行生化、药敏和16SrRNA测序鉴定。结果在所采集的样品中共分离和检测到可培养的16个科32个属56种细菌及10个属的11种真菌;以肠杆菌属、葡萄球菌属、链球菌属和支原体属的携带率最高。其中常见病原菌有甲型副伤寒沙门氏菌、金黄色葡萄球菌、嗜肺巴斯德杆菌、肺炎链球菌、空肠弯曲菌、弗劳地柠檬酸杆菌、鲍氏志贺菌和支原体等。结论树鼩携带细菌和真菌的属种丰富、复杂,存在着多种人兽共患疫病的病原微生物。
OBJECTIVE: To conduct a preliminary investigation of the culturable microbial flora carried by tree shrews and provide the basis for establishing the microbiology grading standards of tree shrews. Methods Totally 61 wild tree shrews were collected for respiratory secretions, intestinal contents and body surface hair. The cells were inoculated on various mediums for bacterial isolation and culture, and biochemical, drug susceptibility and 16S rRNA sequencing were identified. Results A total of 16 cultivars, 16 genera, 56 genera, and 10 genera, 11 fungi were isolated and detected in the collected samples. The carrier rates of Enterobacter, Staphylococcus, Streptococcus and Mycoplasma highest. Among the common pathogens are Salmonella paratyphi A, Staphylococcus aureus, Pneumococcus pneumoniae, Streptococcus pneumoniae, Campylobacter jejuni, Citronella freundii, Shigella flexneri and Mycoplasma and others. Conclusion Tree species are rich in bacteria and fungi species, complex, there are a variety of zoonotic pathogenic microorganisms.