目的　探讨新生儿感染致病菌的流行病学及常见抗菌药物的敏感性。方法　按《全国临床检验操作规程》鉴定细菌 ,纸片扩散法进行药敏试验。结果　 12年内分离出病原菌 2 2 44株。病原菌为 :院外感染前 3位是表皮葡萄球菌、腐生葡萄球菌及大肠埃希菌 ;院内感染前 3位是大肠埃希菌、克雷伯菌及绿脓杆菌。多数院内感染菌株较院外感染菌株对青霉素、氨苄青霉素等 15种常用抗生素的耐药率有不同程度的增加 ,部分差异有统计学意义。总敏感率为 :环丙沙星 95 2 %、诺氟沙星78 8% ,阿米卡星 78 2 %、亚胺培南 +西司他丁 87 7%、万古霉素 81 8%、头孢类抗生素尤其是第 3代头孢菌素 6 0 7%～ 78 1%。结论　院外感染致病菌以革兰阳性球菌为主 ,而院内感染致病菌以革兰阴性杆菌为主 ,后者对许多抗菌药物更易产生耐药性 ;环丙沙星、诺氟沙星、阿米卡星、亚胺培南 +西司他丁、万古霉素及头孢类抗生素尤其是第 3代头孢菌素敏感率均较高 Objective To investigate the epidemiology of neonatal pathogens and the sensitivity of common antimicrobial agents. Methods According to “National Clinical Laboratory Procedures” identification of bacteria, disk diffusion method for susceptibility testing. Results A total of 2244 pathogens were isolated in 12 years. Pathogenic bacteria: the first three out of hospital infections are Staphylococcus epidermidis, Staphylococcus saprophyticus and Escherichia coli; the top three Nosocomial infections are Escherichia coli, Klebsiella and Pseudomonas aeruginosa. In most of the nosocomial infection strains, the resistance rates to 15 commonly used antibiotics, such as penicillin and ampicillin, increased to some extent, some of them were statistically significant. The overall sensitivity was 95 2% for ciprofloxacin, 78 8% for norfloxacin, 78 2% for amikacin, 87 7% for imipenem + cilastatin, 81 8% for vancomycin, Class antibiotics, especially the third generation cephalosporins 607% ~ 78 1%. Conclusion Gram-positive cocci are the main pathogens causing nosocomial infections, while Gram-negative bacilli are the main pathogenic bacteria in nosocomial infections. The latter are more susceptible to many antibacterial drugs. Ciprofloxacin, norfloxacin, Amikacin, imipenem + cilastatin, vancomycin and cephalosporins antibiotics, especially the third-generation cephalosporins susceptibility were high